Buffered Sodium Chloride Peptone Solution pH 7.0 Himedia 500gm

Ingredients                                                         Gms / Litre

Potassium dihydrogen phosphate                     3.600
Disodium hydrogen phosphate dihydrate          7.200
Sodium chloride                                                 4.300
HMC Peptone #                                                 1.000
Final pH ( at 25°C)                                             7.00

Directions
Suspend 14.64 grams (the equivalent weight of dehydrated medium per litre) in 1000 ml purified /distilled water. Heat if
necessary to dissolve the medium completely. For preparation of nonfatty products insoluble in water, add 0.1% w/v
Polysorbate 80 to assist the suspension of poorly wettable substances. Dispense in tubes or flasks or as desired and sterilize
by autoclaving at 15 lbs pressure 121°C for 15 minutes or as per validated cycle.