Product Description
Silica Gel HF254 is a high-purity silica gel specially formulated for Thin Layer Chromatography (TLC) applications. It combines silica gel with calcium sulfate binder (H type) and a fluorescent indicator (F254) that allows compounds to be visualized under UV light at 254 nm.
This formulation ensures excellent separation efficiency, strong adhesion, and reproducible Rf values, making it an ideal choice for pharmaceutical, chemical, and research laboratories conducting routine and advanced chromatographic analyses.

Key Features
- Chemical Name: Silica Gel with Calcium Sulfate Binder & Fluorescent Indicator (HF254)
• Chemical Formula: SiO₂ • nH₂O (with ~13–15% CaSO₄ and UV indicator)
• CAS Number: 112926-00-8
• Appearance: White to off-white fine powder, fluoresces green under UV light (254 nm)
• Particle Size: ~5–40 µm (ideal for uniform TLC coating)
• Binder Content: ~13–15% calcium sulfate (CaSO₄)
• Surface Area: ~500–600 m²/g
• pH (Slurry in water): 6.5–7.5 (neutral to slightly acidic)
• Purity: Analytical grade, designed for accurate TLC analysis
Applications
Laboratory & Research
- Preparation of TLC plates with fluorescent indicator for UV detection (254 nm).
• Identification and purity testing of compounds in pharmaceutical and chemical research.
• Applied in organic synthesis, natural product studies, and phytochemical analysis.
• Suitable for academic, forensic, and industrial laboratories for compound identification and purity checks.
Industrial Uses
- Widely used in pharmaceutical, chemical, food, and herbal industries for quality control and R&D.
• Ideal for monitoring reaction progress and separating intermediates in chemical synthesis.
• Used in analytical laboratories for drug development and compound profiling.
Safety & Handling
- Hazard: Non-toxic and inert; fine dust may irritate eyes, skin, or respiratory system.
• Precautions: Use gloves, goggles, and a dust mask during handling.
• Storage: Store in a tightly sealed container, protected from moisture and direct sunlight.
• Disposal: Dispose of according to local chemical waste management regulations.
Why Choose Silica Gel HF254 for TLC?
- Contains a fluorescent indicator for UV visualization at 254 nm.
• Offers excellent plate adhesion and consistent Rf values.
• Ensures sharp separations with high resolution and reproducibility.
• Preferred adsorbent for TLC analysis in pharmaceutical and chemical laboratories.
• Trusted for routine quality control and advanced analytical applications.
Price in Bangladesh
Available in 100 g, 250 g, 500 g, 1 kg, and bulk packs at competitive market rates.
Special bulk discounts for universities, research labs, and pharmaceutical industries.
Where to Buy
Silica Gel HF254 for Thin Layer Chromatography is available from certified chemical and laboratory reagent suppliers across Bangladesh.
Why Buy from M/S Bright Scientific Co.?
- Reliable Supplier for academic, pharmaceutical, and industrial laboratories.
• Guaranteed Quality & Purity – tested and certified for TLC applications.
• Affordable Pricing for small and bulk quantities.
• Nationwide Delivery – prompt, safe, and dependable service.
Contact Information
M/S Bright Scientific Co.
📞 Phone: 01711285976







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