Product Description: Lactobacillus MRS Broth, Granulated 500Gm

Lactobacillus MRS Broth, Granulated (MRS Broth, Granulated) 500Gm is a dehydrated granulated microbiological culture medium specially formulated for the cultivation of Lactobacillus species. Based on the formulation of de Man, Rogosa and Sharpe (MRS), this medium provides a nutrient-rich environment that supports the growth of Lactobacilli from a variety of clinical and non-clinical sources.

The product is available as HiMedia GM369-500G and is supplied in a convenient 500Gm pack. According to the manufacturer’s technical information, the recommended concentration is 55.15 g/L, allowing approximately 9.07 liters of culture medium to be prepared from 500Gm of dehydrated medium.

Key Features

• Product Name: Lactobacillus MRS Broth, Granulated
• Alternative Name: MRS Broth, Granulated
• Product Code: GM369
• Pack Size: 500Gm
• Product Type: Granulated Dehydrated Culture Medium
• Physical Form: Granules
• Recommended Use: Cultivation of Lactobacillus species
• Concentration: 55.15 g/L
• Approximate Yield: 9.07 L per 500Gm
• Final pH: 6.5 ± 0.2 at 25°C
• Brand: HiMedia
• Origin: India

Composition

The MRS Broth formulation contains several nutrients and selective components that support Lactobacillus cultivation. The composition per liter includes:

• Proteose Peptone: 10.000 g/L
• HM Peptone B: 10.000 g/L
• Yeast Extract: 5.000 g/L
• Dextrose (Glucose): 20.000 g/L
• Polysorbate 80 (Tween 80): 1.000 g/L
• Ammonium Citrate: 2.000 g/L
• Sodium Acetate: 5.000 g/L
• Magnesium Sulphate: 0.100 g/L
• Manganese Sulphate: 0.050 g/L
• Dipotassium Hydrogen Phosphate: 2.000 g/L

The formulation is designed to provide nitrogenous nutrients, vitamins, fermentable carbohydrate, fatty acids, minerals, and buffering components required for the growth of Lactobacilli.

Applications

Lactobacillus Cultivation

Lactobacillus MRS Broth is primarily recommended for the cultivation of Lactobacillus species. It can support growth from different sample sources, including food, dairy products, oral samples, and other microbiological specimens.

Food & Dairy Microbiology

MRS Broth is widely relevant to microbiological research involving food and dairy samples, particularly where Lactobacillus and other lactic acid bacteria are being investigated.

Clinical & Microbiological Research

The medium can be used for laboratory cultivation and research involving Lactobacilli from appropriate clinical and non-clinical samples.

Probiotic & Lactic Acid Bacteria Research

Because Lactobacilli are important microorganisms in food microbiology and probiotic-related research, MRS Broth can be used as a growth medium in laboratory studies involving these organisms.

Research & Educational Laboratories

The granulated formulation is suitable for microbiology laboratories, universities, research institutes, food-testing laboratories, dairy laboratories, and pharmaceutical or biotechnology research facilities.

Principle of MRS Broth

Lactobacillus MRS media are based on the formulation developed by de Man, Rogosa and Sharpe, with modifications used in the manufacturer’s formulation. Proteose peptone and HM Peptone B provide nitrogenous and carbonaceous nutrients, while yeast extract supplies vitamins and other growth factors. Dextrose acts as a fermentable carbohydrate and energy source. Polysorbate 80 provides fatty acids required for Lactobacillus metabolism. Sodium acetate and ammonium citrate help inhibit Streptococci, moulds, and various other microorganisms, supporting the selective cultivation of Lactobacilli.

Preparation & Directions

For preparation, 55.15 grams of the granulated medium should be suspended in 1000 mL of purified or distilled water. Heat may be applied if necessary to dissolve the medium completely. The prepared medium can then be distributed into suitable tubes, bottles, or flasks.

For sterilization, the technical instructions specify autoclaving at 15 lbs pressure (121°C) for 15 minutes. Preparation and sterilization should be performed by trained laboratory personnel following appropriate microbiological procedures.

Storage

The dehydrated medium should be stored under the manufacturer’s recommended conditions. Technical information recommends storing the dehydrated product in a dry environment at 2–8°C and keeping the container tightly closed after opening to help prevent moisture absorption and lump formation.

The prepared medium should also be stored according to validated laboratory procedures and used within the recommended period.

Why Choose Lactobacillus MRS Broth, Granulated?

• Designed specifically for Lactobacillus cultivation
• Based on the established MRS formulation
• Granulated form for convenient laboratory preparation
• Suitable for food, dairy, clinical, and microbiological research
• 500Gm pack provides approximately 9.07 liters of prepared medium
• Suitable for research laboratories and microbiology applications
• Manufactured by HiMedia
• Convenient for routine laboratory media preparation

Lactobacillus MRS Broth, Granulated 500Gm Price in Bangladesh

Lactobacillus MRS Broth, Granulated 500Gm is suitable for universities, microbiology laboratories, food and dairy testing laboratories, research institutes, biotechnology laboratories, pharmaceutical organizations, and other professional users.

For current Lactobacillus MRS Broth, Granulated 500Gm price in Bangladesh, stock availability, institutional orders, and bulk requirements, customers can contact M/S Bright Scientific Co.

Frequently Asked Questions (FAQ)

1. What is Lactobacillus MRS Broth, Granulated?

Lactobacillus MRS Broth, Granulated is a dehydrated microbiological culture medium designed for the cultivation of Lactobacillus species.

2. What is the product code?

The product code is GM369. The 500Gm pack is identified as GM369-500G.

3. What is the pack size?

The standard granulated pack is available in 500Gm.

4. How much medium can be prepared from 500Gm?

At the recommended concentration of 55.15 g/L, approximately 9.07 liters of prepared medium can be produced from a 500Gm pack.

5. What is MRS Broth used for?

It is primarily used for the cultivation of Lactobacillus species and related microbiological research.

6. What is the final pH of the medium?

The final pH is 6.5 ± 0.2 at 25°C.

7. How is MRS Broth prepared?

Suspend 55.15Gm in 1 liter of purified or distilled water, heat if necessary for complete dissolution, distribute into suitable containers, and sterilize according to the manufacturer’s instructions.

8. What is the sterilization condition?

The technical instructions specify sterilization by autoclaving at 121°C for 15 minutes at 15 lbs pressure.

9. What microorganisms is MRS Broth designed for?

It is specifically recommended for the cultivation of Lactobacillus species and supports their growth from various clinical and non-clinical sources.

10. Who can use Lactobacillus MRS Broth?

It is suitable for trained personnel in microbiology, food and dairy testing, biotechnology, pharmaceutical research, academic, and research laboratories.

Contact Information

M/S Bright Scientific Co.
Dhaka, Bangladesh
Phone: 01711285976