MacConkey Broth

Ingredients	Gms / Litre
Gelatin peptone#	20.000
Lactose monohydrate	10.000
Dehydrated bile##	5.000
Bromo cresol purple	0.010
pH after sterilization ( at 25°C)	7.3±0.2

 

**Formula adjusted, standardized to suit performance parameters

# Pancreatic digest of gelatin

## Equivalent to Dehydrated Ox-bile

 

Directions

Suspend 34.51 grams (the equivalent weight of dehydrated medium per litre) in 1000 ml purified/ distilled water. Heat if necessaryto dissolve the medium completely. Dispense into test tubes with inverted Durham tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes or as per validated cycle.