Triple Sugar Iron Agar – 500g is an enriched medium primarily for the differentiation of enteric bacteria based on their ability to ferment sugars and produce gas and hydrogen sulfide. This media is highly beneficial for the detection of *Escherichia coli*, *Salmonella*, *Shigella* species, and other *Enterobacteriaceae*. It is a commonly used technique in clinical microbiology, food safety testing, and environmental microbiology, which is used to detect and differentiate Gram-negative bacteria from clinical, food, and water samples.

Key Features:

– **Differential Medium:** Trypticase Soy Agar with 1% yeast extract, 0.1% each Tween 80 and MgSO4, and 2% NaCl allows bacteria to be differentiated by fermentation of glucose, lactose, and sucrose with production of acid and gas. The use of sodium thiosulfate aids in the identification of hydrogen sulfide production.
– **Colour Reaction:** TSA includes phenol red, a pH indicator that changes from red to yellow when fermentation takes place, and black precipitates are produced when hydrogen sulphide is generated. These color modifications facilitate the identification of bacteria.
– **Widely Used:** Applicable to the isolation and differentiation of intestinal bacteria in clinical, food safety, and other field testing. It aids in the detection of pathogens and distinguishing lactose fermenters, non-fermenters, and hydrogen sulfide producers.
– **Pack Size 500g:** The 500g pack size is well-suited for laboratories with high sample loads or demanding large quantities of medium for routine testing.

Applications:

– **Clinical diagnostics:** TSIA is utilized in clinical microbiology to differentiate enteric pathogens, including *Salmonella*, *Shigella*, and *Escherichia coli*, from stool, urine, blood, and other clinical sources. It assists in the diagnosis of bacterial pathogens causing gastrointestinal and urinary tract infections.
– **Food Safety Testing:** In the food industry, TSA is employed for the detection of bacterial contamination in food products such as meats, dairy products, and vegetables. It is helpful to detect possible foodborne pathogens that would be a public health concern, for food safety and regulatory compliance.
– **Water Analysis:** TSA is also used in environmental microbiology as an indicator of drinking water, wastewater, and recreational waters to determine the presence of bacteria. It is essential for tracking the purity of water, which can aid in the treatment of waterborne diseases.
– **Microbiological studies:** Bacterial fermentation patterns and species identification of *Enterobacteriaceae*. TSA is used. This method is also used in studies on antimicrobial resistance and virulence factors of pathogenic bacteria.

Why Buy Triple Sugar Iron Agar – 500g from Bright Scientific?

– **Durable and Dependable:** Triple Sugar Agar is designed for long shelf life and to deliver reliable results, ensuring it is ideal for use by microbiologists and researchers. The medium enables the reliable differentiation of bacterial fermentation and hydrogen sulphide production.
– **Simple interpretation of the results:** Due to the color changes in the medium (yellow for acid production and dark for the hydrogen sulfide production), the interpretation of the results is straightforward, which can be helpful for non-professional users. This makes identification easier, particularly in diagnostic laboratories with high throughput.
– **Reliable HIMEDIA Name:** A world leader in culture media, including microbiological culture media, known for premium quality. HIMEDIA also provides consistently high-quality media to laboratories in over 100 countries.
– **Affordable Pack:** At 500g per pack, this pack size is suitable for large volume testing laboratories, providing an inexpensive solution with no compromise on quality. It minimizes the need for frequent re-ordering and is a good value for laboratories with high sample volumes.
– **High Quality:** We answer your questions from the moment you place your order, and for years to come, you will get excellent support. Our expert team is ready to help you with troubleshooting, product details, lab application, and general laboratory inserts for your daily needs.

Conclusion:

Triple Sugar Iron Agar – 500g is an indispensable medium for differentiation and identification of enteric bacteria (*Salmonella*, *Shigella*, and *Escherichia coli*). Due to its capacity to recognize sugar fermentations and hydrogen sulfide production, it is an established testing device in clinical diagnostics, food safety testing, and environmental microbiology. Triple Sugar Iron Agar of HIMEDIA provides uniform and excellent growth; the 500g packing is convenient for high-quantity users. The Bright Scientific provides this product at a low price, but with professional advice to get reliable and precise testing.

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