Buffered Sodium Chloride Peptone Solution pH 7.0 HiMedia – 500 g

Product Overview

Buffered Sodium Chloride Peptone Solution pH 7.0 HiMedia – 500 g is a dehydrated laboratory medium designed for microbiological testing, sample dilution, and the preparation of microbial test suspensions. Manufactured by HiMedia Laboratories, it is recommended for microbial limit testing of pharmaceutical products in accordance with harmonized USP, EP, BP, JP, and IP methodologies.

This specialized formulation combines phosphate buffers, sodium chloride, and peptone to help maintain a stable pH and support the viability of microorganisms during sample preparation. Its balanced composition makes it useful in pharmaceutical quality control laboratories, microbiology research facilities, and other professional testing environments.

The medium is supplied as a dehydrated powder in a 500 g pack. It must be dissolved in purified or distilled water and sterilized according to the manufacturer’s instructions before use. It is intended primarily as a diluent and for preparing microbial test suspensions, rather than as a general-purpose growth medium.

Product Specifications

• Product Name: Buffered Sodium Chloride Peptone Solution pH 7.0
• Brand: HiMedia
• Pack Size: 500 g
• Final pH: 7.00 at 25°C
• Physical Form: Dehydrated powder
• Application: Microbial limit testing and preparation of test suspensions
• Recommended Standards: USP, EP, BP, JP, and IP harmonized methodologies

Composition per Litre

The manufacturer’s standard formulation contains:

• Potassium Dihydrogen Phosphate: 3.600 g/L
• Disodium Hydrogen Phosphate Dihydrate: 7.200 g/L
• Sodium Chloride: 4.300 g/L
• HMC Peptone: 1.000 g/L

The formula is adjusted and standardized by the manufacturer to meet the required performance parameters. HMC Peptone is equivalent to peptone derived from meat or casein.

Applications and Benefits

1. Pharmaceutical Microbial Limit Testing: Recommended as a diluent for microbiological examination of pharmaceutical products using harmonized testing methods.

2. Preparation of Microbial Test Suspensions: Helps prepare standardized microbial suspensions for validating microbiological testing procedures.

3. Sample Dilution: Used to dilute or dissolve suitable water-soluble and non-fatty, water-insoluble product samples during microbiological testing.

4. Microbiology Research: Suitable for professional laboratories conducting microbial recovery studies and related analytical procedures.

5. pH and Osmotic Balance: Phosphate salts help maintain the required pH, while sodium chloride supports osmotic balance and cellular integrity. Peptone provides nutrients that help maintain microbial viability.

Preparation Instructions

Suspend 14.64 g of dehydrated medium in 1,000 mL of purified or distilled water. Heat if necessary to dissolve the powder completely. For non-fatty products that are insoluble in water, the manufacturer specifies adding 0.1% w/v Polysorbate 80 when appropriate to assist suspension.Buffered Sodium Chloride

Dispense the prepared medium into suitable tubes or flasks and sterilize by autoclaving at 121°C under 15 lb pressure for 15 minutes, or follow a validated sterilization cycle. Always consult the current manufacturer’s technical data sheet and the applicable testing procedure before preparation.Buffered Sodium Chloride

Storage and Safety

Store the dehydrated medium in a tightly closed container under the manufacturer’s recommended conditions, generally between 10°C and 30°C. Protect it from moisture, excessive heat, and direct sunlight. Close the container securely after opening to prevent moisture absorption and powder clumping.

Wear appropriate laboratory protective equipment and follow good microbiological laboratory practices. Used media and contaminated materials must be decontaminated and disposed of according to established laboratory procedures and applicable regulations.Buffered Sodium Chloride

Frequently Asked Questions (FAQ)

1. What is Buffered Sodium Chloride Peptone Solution pH 7.0?

It is a dehydrated microbiological medium used mainly for sample dilution, microbial limit testing, and preparation of test suspensions.Buffered Sodium Chloride

2. What is the pack size?

This product is supplied in a 500 g pack.

3. What is the final pH of the prepared medium?

The specified final pH is 7.00 at 25°C.

4. How much powder is required per litre?

Use 14.64 g of dehydrated medium per 1,000 mL of purified or distilled water.Buffered Sodium Chloride

5. Is this product ready to use?

No. The 500 g product is dehydrated powder that must be dissolved and sterilized according to the manufacturer’s instructions.Buffered Sodium Chloride

6. Which standards is it recommended for?

It is recommended for harmonized pharmaceutical microbiological testing methods associated with USP, EP, BP, JP, and IP.Buffered Sodium Chloride

7. Where can I buy this product in Bangladesh?

Contact Bright Scientific to confirm current stock, pricing, and delivery availability in Bangladesh.Buffered Sodium Chloride