Product Description Kligler Iron Agar  

Kligler Iron Agar (KIA) 500g is a differential medium designed for the identification and differentiation of enteric bacteria. It is an essential test in a microbiological laboratory for identifying bacterial fermentation patterns, hydrogen sulfide (H2S) production, and the fermentation of glucose and lactose. This medium is instrumental in the ability to recognise a variety of enteric bacteria, including pathogenic ones such as Escherichia coli, Salmonella, and Shigella. The medium was prepared to demonstrate the ability of bacteria to ferment glucose and/or lactose, and to reduce sulfur compounds to elemental sulfur; crucial metabolic features were monitored.
Both glucose and lactose are present in fermentable carbohydrates, sodium thiosulfate provides sulfur, and phenol red indicates the pH of the medium in the Kligler Iron Agar. In clinical diagnostics, this medium is used to distinguish between enteric bacteria based on their metabolism, allowing for a more precise diagnosis of pathogens and helping in the administration of appropriate treatment. The production of hydrogen sulfide, indicated by black precipitates in the medium, is an additional way to distinguish between sulfur-reducing bacteria.

 

Key Features : Kligler Iron Agar

Fermentation of Glucose and Lactose: The medium contains both glucose and lactose, which helps distinguish between bacteria that can ferment these sugars. The pH indicator (phenol red) turns from red to yellow in acidic products produced by fermentation, making fermenters easy to recognise.
Hydrogen Sulfide Production: The medium includes sodium thiosulfate as a source of sulfur, enabling the detection of H2S production. Bacteria that reduce thiosulfate to H2S will produce a black precipitate in the medium, a characteristic that identifies some enteric bacteria, including Salmonella.
PH Indicator: Phenol red is added to detect acid production because of the fermentation of glucose and lactose. Yellow colour denotes that acid (usually through fermentation) has been produced, while a red or neutral pH results in the medium turning red. This characteristic makes it valuable in differentiating bacteria that ferment glucose or lactose from those that do not.
Selective and Differential: KIA is a selective and differential medium because it is capable of identifying enteric bacteria and isolating one species from another based on their ability to ferment glucose and/or lactose and produce H2S.
Superior Performance: Kligler Iron Agar is specially formulated for accurate and reproducible results on a broad range of applications. The medium provides discriminating results, up to the level of metabolic patterns in organisms, offering precise and rapid identification of microbes.
Quality: The agar base is made with high-quality ingredients, guaranteeing the desired consistency and accuracy. The medium is free of non-bacterial impurities, thereby meeting high requirements for bacteriological purposes.

Specifications: Kligler Iron Agar

Cat No: Kligler Iron Agar 500g
Amount: 500g (Dehydrated powder)
Storage: Store in a cool, dry area, out of direct sunlight and humidity. Once the complex is formed, the medium can be stored at 4 °C for a short-term period.
Application: For isolation and differentiation of enteric bacteria based on carbohydrate fermentation and hydrogen sulphide production.
pH: Usually about 7.2 ± 0.2
Preparation: To prepare, dissolve the powder in distilled water. Boil for complete dissolution, autoclave 121°C/15 min, then cool to 45-50°C before use.

Applications : Kligler Iron Agar

Water Testing: KIA is recommended for use as a test medium for detecting and enumerating enteric bacteria in water samples, including those from natural and treated environmental sources, for recreational water and environmental water quality testing. Enteric bacteria, such as E. coli and Salmonella, are indicators of faecal contamination, so this medium is useful as a growth medium in water quality assessment.
Food Microbiology: This medium is commonly used in the food industry for the isolation and identification of enteric pathogens that may contaminate food during processing or due to poor handling. It’s especially valuable in identifying Salmonella and Shigella, two leading causes of food poisoning.
Clinical Microbiology: KIA is indispensable in clinical diagnostic laboratories for the isolation and differentiation of enteric pathogens from stool, urine, blood, and other body fluids. It is helpful in the diagnosis of Salmonella, Shigella and E. coli infections, and others.
Environmental Monitoring: KIA is also used in environmental microbiology to detect contamination in soil, wastewater, and other environmental samples. This app’s purpose is to help you discover and avoid where microbial infections occur in public, and you help keep public places disease-free.

Why is Kligler Iron Agar 500g from Bright?

Premium Ingredients: Towards a more consistent, high-quality performance, it is made with premium ingredients.
Practical to apply to a variety of microbiological tests such as clinical diagnosis, food detection, water quality testing and environmental detection.
Trustworthy Supplier: Bright Scientific fully guarantees the authenticity of products for all your lab needs.
Quick Shipping: We offer timely shipping to help keep your research or product program on track.

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