Nutrient Agar 500gm HiMedia – M001

HiMedia Nutrient Agar 500gm (M001) is a general-purpose, non-selective microbiological culture medium formulated for the cultivation and maintenance of less fastidious microorganisms. It is widely used in microbiology laboratories for routine culture, microbial maintenance, enumeration, educational purposes, and research applications.

The medium contains Peptone, HM Peptone B, Yeast Extract, Sodium Chloride, and Agar, providing essential nutrients required for the growth of a wide range of microorganisms. The standardized formulation provides a firm gel suitable for routine microbiological culture techniques.

HiMedia Nutrient Agar M001 is suitable for use in academic, research, clinical, food and dairy, water testing, pharmaceutical, and industrial microbiology laboratories.

Key Features

• General-purpose, non-selective microbiological culture medium
• Suitable for cultivation of less fastidious microorganisms
• HiMedia product code M001
• Provides essential nutrients for routine microbial cultivation
• Suitable for maintenance and propagation of microbial cultures
• Firm gel formation for standard culture applications
• Suitable for laboratory research, teaching, and routine microbiological work
• Can be used as a base medium for specific laboratory applications
• Convenient 500gm pack size

Applications

HiMedia Nutrient Agar M001 is commonly used for:

• Routine cultivation of microorganisms
• Maintenance of bacterial cultures
• Microbiological research
• Educational and teaching laboratories
• Purity checking of microbial cultures
• Food and dairy microbiological testing
• Water microbiological analysis
• Pharmaceutical microbiology
• Industrial microbiology
• General laboratory culture applications

Composition

Component	Quantity
Peptone	5.0 g/L
Sodium Chloride	5.0 g/L
HM Peptone B	1.5 g/L
Yeast Extract	1.5 g/L
Agar	15.0 g/L
Final pH at 25°C	7.4 ± 0.2

Preparation

For routine preparation, suspend 28.0gm of Nutrient Agar in 1000mL of purified or distilled water. Heat with frequent agitation until the medium is completely dissolved. Sterilize by autoclaving at 121°C for 15 minutes or according to the manufacturer’s validated instructions.

After sterilization, allow the medium to cool to the appropriate temperature before dispensing into sterile Petri plates or other suitable laboratory containers.

Preparation and sterilization should always be carried out according to the manufacturer’s current technical documentation and established laboratory procedures.

Physical Characteristics

The dehydrated medium is supplied as a cream to yellow, homogeneous, free-flowing powder. When prepared, it forms a light-yellow, clear to slightly opalescent gel with firm gelling characteristics.

The specified final pH of the prepared medium is 7.4 ± 0.2 at 25°C.

Product Specifications

Product Name: Nutrient Agar
Brand: HiMedia
Product Code: M001
Product Type: Microbiological Culture Medium
Medium Type: General Purpose, Non-Selective
Pack Size: 500gm
Preparation: 28.0 g/L
Final pH: 7.4 ± 0.2 at 25°C
Physical Form: Dehydrated Powder
Application: Microbiological Culture and Laboratory Research
Origin: India

Storage

Store the dehydrated medium in a tightly closed container in a cool, dry place, away from moisture and direct contamination. Always follow the storage conditions, shelf-life, and handling instructions provided on the manufacturer’s label and technical documentation.

Laboratory Use

This product is intended for laboratory and professional microbiological use. Appropriate laboratory safety practices should be followed when preparing and handling culture media or working with microorganisms.

Users should follow applicable microbiological procedures, safety protocols, and the manufacturer’s instructions during preparation and use.

Package Includes

• 1 × HiMedia Nutrient Agar M001 – 500gm

Why Choose HiMedia Nutrient Agar M001?

HiMedia Nutrient Agar M001 provides a standardized general-purpose medium for routine microbiological applications. Its balanced nutrient composition and reliable gel formation make it suitable for laboratories requiring a versatile medium for the cultivation and maintenance of less fastidious microorganisms.

It is an appropriate choice for universities, research laboratories, clinical laboratories, food and dairy testing facilities, pharmaceutical laboratories, water testing laboratories, and industrial microbiology facilities.

Legal Disclaimer

Product packaging, labeling, batch number, and appearance may vary depending on the manufacturing batch. Always refer to the manufacturer’s current product label, technical data sheet, and safety documentation before preparation or use.

For laboratory use only. Not for human or animal consumption.

Supplier

M/S Bright Scientific Co. supplies laboratory chemicals, microbiological culture media, scientific instruments, laboratory glassware, and laboratory consumables across Bangladesh.

Contact: +8801711285976
Business Hours: 10AM – 8PM (Friday OFF)