Phenol:Chloroform: Isoamyl Alcohol (25:24:1) pH 8.0 for Molecular Biology – 500ml

Phenol:Chloroform: Isoamyl Alcohol (25:24:1) pH 8.0 for Molecular Biology is a laboratory chemical reagent designed for nucleic acid extraction and purification procedures. This solution contains phenol, chloroform, and isoamyl alcohol in a 25:24:1 ratio and is formulated at approximately pH 8.0. It is used in molecular biology laboratories for separating DNA from proteins and other cellular contaminants during appropriate extraction procedures.

This reagent is suitable for research laboratories, biotechnology facilities, universities, academic institutions, and other scientific laboratories that perform molecular biology experiments. Its formulation supports phase separation during DNA purification, helping researchers obtain nucleic acid preparations for subsequent laboratory analysis.

Product Information

• Product Name: Phenol:Chloroform: Isoamyl Alcohol (25:24:1) pH 8.0 for Molecular Biology
• Product Code: 69031
• CAS No.: 136112-00-0
• Chemical Composition: Phenol, Chloroform, and Isoamyl Alcohol
• Mixing Ratio: 25:24:1
• pH: Approximately 8.0
• Appearance: Clear, pale yellow liquid
• Stabilizer: 0.1% 8-Hydroxyquinoline
• DNase: Not detected
• RNase: Not detected
• Protease: Not detected
• Formula: Not applicable to the mixture as a whole
• Pack Size: 500ml
• Brand: SRL (Sisco Research Laboratories Pvt. Ltd.)
• Origin: India
• Product Category: Laboratory Chemicals / Molecular Biology Reagents

What Is Phenol:Chloroform Alcohol (25:24:1) pH 8.0?

Phenol:Chloroform: Isoamyl Alcohol (25:24:1) pH 8.0 for Molecular Biology is a premixed organic solvent reagent used in nucleic acid purification. It combines three components with complementary functions during extraction.

Phenol helps denature proteins and disrupt protein structures. Chloroform assists organic and aqueous phase separation and helps remove proteins and lipids from the sample. Isoamyl alcohol helps reduce foaming and supports easier handling during mixing and separation.

When used in a suitable extraction protocol, the reagent helps separate nucleic acids from unwanted cellular materials. Under appropriate alkaline-pH conditions, DNA is generally recovered from the aqueous phase, while many proteins and other contaminants remain in the organic phase or at the interface. The final result depends on the sample type, extraction conditions, and the laboratory protocol used.

Principle of Phenol:Chloroform: Isoamyl Alcohol (25:24:1) pH 8.0 for Molecular Biology Extraction

The extraction principle is based on the different chemical properties of nucleic acids, proteins, and organic solvents. During the extraction procedure, phenol denatures proteins, while chloroform supports separation between the aqueous and organic phases. Isoamyl alcohol reduces foaming during mixing.

Following the prescribed mixing and centrifugation steps, the sample separates into distinct phases. Under suitable conditions, DNA remains in the aqueous phase, whereas many proteins, lipids, and other contaminants are transferred to the organic phase or interphase.

The aqueous phase can then be collected for subsequent purification or analysis according to the validated laboratory procedure. Careful phase separation is important to reduce contamination and improve the quality of the resulting nucleic acid preparation.

Applications of Phenol:Chloroform: Isoamyl Alcohol (25:24:1) pH 8.0 for Molecular Biology

This reagent is commonly used in molecular biology and related research applications, including:

• DNA extraction and purification
• Separation of nucleic acids from proteins and cellular contaminants
• Molecular biology research
• Biotechnology and genetic research
• Preparation of DNA samples for downstream analysis
• Research involving cellular and biological samples
• Academic and university laboratory experiments
• Scientific research and development
• Suitable nucleic acid purification protocols

The pH 8.0 formulation is generally associated with DNA extraction procedures. RNA extraction may require a specifically formulated acidic reagent, depending on the method. Always select the reagent according to the intended application and the manufacturer’s instructions.

Product Quality and Laboratory Suitability

The supplied product specifications indicate that DNase, RNase, and protease are not detected. This is relevant to molecular biology applications where nucleic acid integrity and the minimization of enzymatic contamination are important.

The solution is supplied as a ready-prepared reagent with a specified mixing ratio and pH. Before use, laboratories should confirm the product identity, batch details, certificate of analysis, and current Safety Data Sheet (SDS).

Actual suitability depends on the requirements of the experiment and the laboratory’s validated procedures.

Frequently Asked Questions (FAQ)

1. What is Phenol:Chloroform: Isoamyl Alcohol (25:24:1) pH 8.0 for Molecular Biology used for?

It is used primarily in suitable DNA extraction and purification procedures to separate nucleic acids from proteins and other cellular contaminants.Phenol:Chloroform: Isoamyl Alcohol

2. What does the 25:24:1 ratio mean?

The ratio represents the formulation proportions of phenol, chloroform, and isoamyl alcohol, respectively.

3. What is the pH of this reagent?

The specified pH is approximately 8.0.

4. What is the pack size?

The supplied pack size is 500ml.

5. What is the product code?

The product code provided is 69031.

6. Which brand manufactures this product?

The supplied information identifies SRL (Sisco Research Laboratories Pvt. Ltd.) as the brand.Phenol:Chloroform: Isoamyl Alcohol

7. Is this reagent suitable for DNA extraction?

It is intended for suitable molecular biology extraction procedures, including DNA purification, when used according to the relevant protocol.

8. Can this product be used for RNA extraction?

The appropriate formulation depends on the extraction method. A pH 8.0 formulation is generally associated with DNA extraction; RNA extraction may require an acidic formulation or a different validated reagent.

9. What is the appearance of this product?

The supplied specifications describe it as a clear, pale yellow liquid.Phenol:Chloroform: Isoamyl Alcohol

10. Does the product contain DNase, RNase, or protease?

The supplied specifications state that DNase, RNase, and protease are not detected. Refer to the batch-specific documentation for confirmation.

11. What safety precautions are required?

Use a chemical fume hood, suitable chemical-resistant PPE, and the current SDS. The product should be handled only by trained laboratory personnel.Phenol:Chloroform: Isoamyl Alcohol

12. Where can I purchase this reagent in Bangladesh?

Contact M/S Bright Scientific Co. to inquire about availability, current pricing, and quotations for the 500ml pack.Phenol:Chloroform: Isoamyl Alcohol

WhatsApp: +880 1711-285976

LABORATORY USE ONLY, NO ANOTHER USE.