Potato Dextrose Broth, Granulated – 500gm
Potato Dextrose Broth, Granulated 500gm is a microbiological culture medium manufactured by HiMedia, India. It is recommended for the cultivation of yeasts and molds and is widely used in microbiological testing of food and dairy products, fungal research, and laboratory applications. The medium contains potato infusion and dextrose, which support luxuriant fungal growth.
Product Overview
Potato Dextrose Broth is a dehydrated granulated culture medium designed for preparing a liquid medium for fungal cultivation. It is suitable for laboratory studies involving yeast and mold growth, fungal sporulation, maintenance of selected dermatophyte stock cultures, and differentiation of certain dermatophytes based on pigment production.
The medium can also be acidified with sterile tartaric acid when a lower pH is required to inhibit bacterial growth. Follow the manufacturer’s instructions and the requirements of the specific test method.
Key Features
- Product Name: Potato Dextrose Broth, Granulated
- Brand: HiMedia
- Pack Size: 500gm
- Origin: India
- Physical Appearance: Off-white to yellow-colored granulated medium
- Typical pH: 5.1 ± 0.2 at 25°C
- Primary Application: Cultivation of yeasts and molds
- Suitable for: Microbiology, food testing, dairy testing, and fungal research laboratories
Principle and Interpretation
Potato infusion provides nutrients that support fungal growth, while dextrose serves as an energy source. Together, these components promote the growth of yeasts and molds under suitable laboratory conditions.
Potato Dextrose Broth is recommended by recognized microbiological methods for the examination of foods and dairy products. It is also used for stimulating sporulation, maintaining stock cultures of certain dermatophytes, and differentiating typical dermatophyte varieties based on pigment production.
When bacterial inhibition is required, the medium may be adjusted to approximately pH 3.5 using sterile 10% tartaric acid, according to the applicable method. The acidified medium should not be heated after the acid has been added.
Directions for Preparation
- Suspend 24.0 grams of granulated medium in 1000 mL of distilled water.
- Heat if necessary to dissolve the medium completely.
- Sterilize by autoclaving at 121°C (15 lbs pressure) for 15 minutes.
- Mix well before dispensing into suitable laboratory containers.
- When a pH of 3.5 is required, add sterile 10% tartaric acid to the sterile, cooled medium. Approximately 1 mL of acid is indicated for every 100 mL of medium.
- Do not heat the medium after adding tartaric acid.
Always follow the product label and the applicable laboratory method when preparing culture media.
Quality Control Specifications
Appearance: Off-white to yellow-colored granular medium.
Color and Clarity of Prepared Medium: Light amber-colored, clear to slightly opalescent solution in tubes.
Reaction: The pH of a 2.4% w/v aqueous solution at 25°C is typically 5.1 ± 0.2.
Acceptable pH Range: 4.90–5.30.
Cultural Response
The following cultural characteristics are reported after incubation at 25–30°C for 4–5 days.
| Test Organism | Inoculum (CFU) | Expected Growth | Ascospore Formation |
|---|---|---|---|
| Aspergillus brasiliensis ATCC 16404 | 50–100 | Luxuriant | Negative |
| Candida albicans ATCC 10231 | 50–100 | Luxuriant | Negative |
| Saccharomyces cerevisiae ATCC 9763 | 50–100 | Luxuriant | Positive |
Note: Aspergillus brasiliensis was formerly known as Aspergillus niger. CFU means colony-forming units.
Storage and Shelf Life
Store the dehydrated medium between 10°C and 30°C in a tightly closed container. Store prepared medium at 2–8°C, as directed by the manufacturer. Use the product before the expiry date printed on the label. Protect the container from moisture and contamination.
Applications
Potato Dextrose Broth, Granulated is used for:
- Cultivation of yeasts and molds.
- Microbiological examination of food and dairy products.
- Fungal growth and sporulation studies.
- Maintenance of selected dermatophyte stock cultures.
- Differentiation of certain dermatophytes based on pigment production.
- Microbiology teaching, research, and quality-control laboratories.
Frequently Asked Questions (FAQ)
1. What is Potato Dextrose Broth used for?
It is primarily used to cultivate yeasts and molds and for selected fungal research and microbiological testing applications.
2. What is the recommended preparation quantity?
The supplied directions specify 24.0 grams of medium per 1000 mL of distilled water.
3. What is the typical pH of Potato Dextrose Broth?
The typical pH is 5.1 ± 0.2 at 25°C, with a stated range of 4.90–5.30.
4. Can the medium be acidified?
Yes. When required by the test method, sterile 10% tartaric acid can be added to the sterile, cooled medium to adjust the pH to approximately 3.5. Do not heat the medium after acidification.
5. How should the product be stored?
Store the dehydrated product at 10–30°C in a tightly closed container. Store prepared medium at 2–8°C and observe the manufacturer’s expiry date.
6. What is the pack size and origin?
This product is supplied in a 500gm pack under the HiMedia brand, with India as its origin.
Order from Bright Scientific
Bright Scientific supplies laboratory chemicals, microbiological culture media, scientific instruments, and laboratory consumables in Bangladesh. Contact our sales team for current pricing, availability, and quotation requests.
LABORATORY USE ONLY, NO ANOTHER USE.
Reviews
There are no reviews yet.