Trypan Blue (Code: 06309) – Biological Stain for Cell Viability Testing & Cell Culture
Product Description
Trypan Blue (Code: 06309) is a premium-quality biological stain and vital dye extensively used for cell viability analysis, cell culture, cytology, histology, and biotechnology research. This highly purified azo dye is designed to distinguish between viable (live) and non-viable (dead) cells by selectively penetrating cells with damaged membranes while being excluded by healthy cells. Because of its accuracy, simplicity, and reproducibility, Trypan Blue has become one of the most widely used reagents for routine cell counting and viability assessment in research and diagnostic laboratories worldwide.
Supplied by M/S Bright Scientific Co., Trypan Blue is manufactured to high laboratory standards, ensuring excellent staining performance, batch-to-batch consistency, and reliable analytical results. It is suitable for universities, research institutes, biotechnology laboratories, pharmaceutical industries, hospitals, diagnostic centers, and quality control laboratories across Bangladesh.
Trypan Blue appears as a dark blue crystalline powder or deep blue aqueous solution and is highly soluble in water, making it convenient for laboratory preparation and routine use. During the Trypan Blue Exclusion Test, viable cells maintain intact cell membranes and therefore exclude the dye, remaining transparent under the microscope. In contrast, dead or damaged cells absorb the dye and appear blue, enabling researchers to accurately determine cell viability and calculate live cell percentages.
The reagent is widely used in mammalian cell culture, microbial research, stem cell studies, immunology, molecular biology, oncology research, toxicology, and pharmaceutical development. Before cell subculturing, cryopreservation, transfection, or downstream molecular experiments, researchers commonly use Trypan Blue to evaluate cell health and ensure experimental accuracy.
Its excellent optical properties and high staining quality provide sharp visualization under light microscopes, making it suitable for routine laboratory examinations, teaching demonstrations, and advanced biological research. Whether used in academic laboratories or industrial biotechnology facilities, Trypan Blue provides consistent staining performance that supports reliable experimental outcomes.
Manufactured with a purity of 98% or higher (Biological Stain Grade), the reagent offers long shelf life and dependable performance when stored properly. It is available as crystalline powder in multiple pack sizes as well as ready-to-use aqueous solutions, allowing laboratories to select the format most suitable for their applications.
Benefits of Using Trypan Blue
Trypan Blue provides a simple, rapid, and cost-effective method for determining cell viability without requiring sophisticated instrumentation. It helps researchers distinguish living and dead cells with excellent accuracy, improving the reliability of cell culture experiments and biological assays. The stain produces consistent microscopic visualization, minimizes experimental variation, and supports reproducible research results.
Because of its dependable staining characteristics, Trypan Blue is widely accepted as the standard reagent for routine cell viability testing in life science laboratories around the world.
Key Features
- Premium Biological Stain
- Product Code: 06309
- Chemical Name: Trypan Blue
- Synonyms: Diamine Blue, Direct Blue 14, Vital Stain Blue
- CAS No.: 72-57-1
- Molecular Formula: C₃₄H₂₈N₆Na₄O₁₄S₄
- Molecular Weight: 960.81 g/mol
- Purity ≥98% (Biological Stain Grade)
- Dark Blue Crystalline Powder
- Water Soluble
- Excellent Cell Viability Indicator
- Ideal for Trypan Blue Exclusion Test
- High Staining Efficiency
- Excellent Microscopic Contrast
- Reliable Batch-to-Batch Consistency
- Suitable for Cell Culture & Cytology
- Long Shelf Life
- Available in Powder & Ready-to-Use Solution
Trypan Blue (Code: 06309) – High-Purity Biological Stain for Cell Viability Testing, Cell Culture, Cytology, Microscopy, and Biotechnology Research.

Applications
Laboratory Applications
- Cell Viability Assay
- Trypan Blue Exclusion Test
- Cell Counting
- Mammalian Cell Culture
- Microbial Cell Culture
- Stem Cell Research
- Cytology
- Histology
- Microscopy
- Immunology Research
- Molecular Biology
- Biotechnology Research
- Pharmaceutical Research
- Toxicology Studies
- Cell Membrane Integrity Analysis
- Biochemistry Laboratories
- Life Science Research
- Quality Control Laboratories
- University Laboratories
- Research Institutes
Industrial Applications
- Pharmaceutical Industries
- Biotechnology Companies
- Clinical Research Laboratories
- Diagnostic Centers
- Hospitals
- Academic Institutions
- Medical Colleges
- Vaccine Research Facilities
- Contract Research Organizations (CROs)
- Biological Product Development
Why Choose Trypan Blue 06309?
- High-purity Biological Stain Grade reagent
- Excellent cell viability determination
- Fast and accurate staining performance
- Outstanding microscopic visibility
- Reliable and reproducible results
- Suitable for research and educational laboratories
- Available in multiple pack sizes
- Long shelf life
- Competitive pricing
- Fast delivery across Bangladesh
Technical Specifications
| Parameter | Details |
|---|---|
| Product Name | Trypan Blue |
| Product Code | 06309 |
| Chemical Name | Trypan Blue |
| CAS Number | 72-57-1 |
| Molecular Formula | C₃₄H₂₈N₆Na₄O₁₄S₄ |
| Molecular Weight | 960.81 g/mol |
| Purity | ≥98% (Biological Stain Grade) |
| Appearance | Dark Blue Crystalline Powder |
| Solubility | Soluble in Water |
| Absorption Maximum (λmax) | Approximately 607 nm |
| Storage Temperature | 15–25°C |
| Application | Cell Viability Testing & Biological Staining |
| Available Pack Sizes | 10 g, 25 g, 100 g, 1% Solution |
Safety & Handling
Precautions
- Wear laboratory gloves, goggles, and protective clothing.
- Avoid inhalation and direct contact with skin or eyes.
- Handle inside a well-ventilated laboratory.
- Wash hands thoroughly after use.
- Keep the container tightly closed when not in use.
Storage Conditions
- Store at room temperature (15–25°C).
- Protect from moisture and direct sunlight.
- Keep containers tightly sealed.
- Store away from incompatible chemicals.
Why Buy from M/S Bright Scientific Co.?
- Genuine laboratory chemicals and biological stains
- Premium quality assurance
- Competitive market prices
- Bulk order facilities
- Fast nationwide delivery across Bangladesh
- Trusted supplier for universities, hospitals, biotechnology companies, pharmaceutical industries, research laboratories, and diagnostic centers
- Professional technical support and customer service
Contact Information
M/S Bright Scientific Co.
📍 32/1 Shahid Nazrul Islam Sharak, Hatkhola Road, Shuvasca Plaza, Tikatuly, Dhaka-1203, Bangladesh
📞 Phone: +880 1711-285976
📧 Email: brightscientific@gmail.com
📧 Email: info@brightscientific.com







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