Trypsin 1:250

Product Description

Trypsin 1:250 (From Porcine Pancreas) (Code: 06311) is a high-quality proteolytic enzyme preparation derived from the pancreas of porcine origin, standardized to a specific enzymatic activity of 1:250 (1 part Trypsin equivalent to 250 parts casein). It is a serine protease that hydrolyzes peptide bonds at the carboxyl side of lysine and arginine residues, making it a key reagent in cell culture, protein biochemistry, and enzymology research.

This form of Trypsin offers moderate enzymatic activity, suitable for routine cell detachment, tissue dissociation, protein digestion, and peptide mapping. It ensures controlled proteolysis without excessive damage to biological materials, making it ideal for academic, pharmaceutical, and biotechnological laboratories.

Key Features

• Product Code: 06311
• Chemical Name: Trypsin (From Porcine Pancreas)
• Enzyme Commission (EC) Number: 3.4.21.4
• Activity: 1:250 (Equivalent to 250 units/mg protein)
• Source: Porcine pancreas
• Appearance: White to pale yellow amorphous or crystalline powder
• Molecular Weight: ~23,800 Da
• Optimum pH: 7.5–8.5
• Optimum Temperature: 37°C
• Solubility: Soluble in distilled water, saline, or phosphate buffer
• Stability: Enhanced by calcium salts or stabilizing agents
• Storage: Store at 2–8°C in tightly sealed containers

Applications

Laboratory & Research

• Cell Culture: Used for gentle detachment of adherent cells during subculturing and passage.
• Protein Digestion: Facilitates controlled hydrolysis of proteins for amino acid sequencing and peptide analysis.
• Tissue Dissociation: Helps isolate viable cells from animal or organ tissues.
• Enzymology Studies: Serves as a standard enzyme in kinetic and structural research.
• Molecular Biology: Used in purification of recombinant proteins and DNA extraction workflows.

Industrial & Institutional Uses

• Pharmaceuticals & Biotech: Applied in enzyme-based formulations, diagnostics, and research reagents.
• Academic Research: Commonly used for teaching enzymatic reactions, proteolysis, and biochemistry.
• Food & Bioprocessing: Supports controlled protein breakdown and analysis.

Safety & Handling

• Hazard: Enzyme dust may cause irritation or allergic sensitization if inhaled or in contact with skin.
• Precautions: Use protective gloves, goggles, and laboratory coat. Handle in a ventilated area or under a fume hood.
• Storage: Store in a cool, dry, tightly closed container between 2–8°C. Protect from moisture and direct sunlight.
• Disposal: Dispose of enzyme waste as biological material in accordance with local regulations.

Price in Bangladesh

Available in 10 g, 25 g, 100 g, and 500 g packs.
Custom bulk quantities and enzyme activity certifications available on request.
Competitive pricing with prompt nationwide delivery.

Why Choose Trypsin 1:250 (From Porcine Pancreas) 06311 from M/S Bright Scientific Co.?

• High-purity enzyme standardized to 1:250 activity.
• Reliable and consistent performance in biochemical and cell culture applications.
• Derived from high-quality porcine pancreatic source.
• Available in both research and bulk-grade packaging.
• Trusted by universities, biotech industries, and research institutions across Bangladesh.

Contact Information

M/S Bright Scientific Co.
📞 Phone: 01711285976