Product Description: Tween 20 Molecular Biology

Tween 20 Molecular Biology is a high-quality non-ionic surfactant widely used in molecular biology, biochemistry, cell biology, microbiology, immunoassay, and laboratory research applications. Also known as Polysorbate 20, Tween 20 is a versatile laboratory reagent valued for its surface-active properties, water solubility, and compatibility with a wide range of biological and biochemical procedures.

With CAS No. 9005-64-5, molecular formula C58H114O26, and molecular weight 1227.72 g/mol, this Tween 20 is supplied in a Molecular Biology grade suitable for laboratory procedures requiring a controlled and reliable non-ionic detergent.

Tween 20 is supplied as a golden yellow, viscous liquid at 20 °C and is soluble in water. Its non-ionic nature makes it useful in applications where mild detergent action is required without introducing ionic charges that can interfere with biological molecules or experimental systems.

Specifications of Tween 20 Molecular Biology

• Product Name: Tween 20 Molecular Biology
• Grade: Molecular Biology
• Chemical Name: Polysorbate 20
• CAS No.: 9005-64-5
• Molecular Formula: C58H114O26
• Molecular Weight: 1227.72 g/mol
• H.S. Code: 3402.4200
• Physical State at 20 °C: Liquid
• Colour: Golden yellow
• pH Value: 7
• Flash Point: 110 °C
• Boiling Point: 100 °C
• Vapour Pressure: < 1.33 hPa at 20 °C
• Density: 1.095 g/cm³
• Solubility in Water: Soluble
• Appearance: Pale yellow colour viscous liquid
• DNAase/RNAase/Proteases: Not detectable
• Density (d20°C/4°C): 1.1–1.11
• Hydroxyl Number: Approx. 100
• Saponification Value: 40–50
• Shelf Life: 60 Months

Tween 20 Molecular Biology for Laboratory Applications

Tween 20 Molecular Biology is commonly used as a non-ionic detergent in biological and biochemical laboratory procedures. Its surfactant properties help reduce surface tension and support effective interaction between aqueous solutions and surfaces.

In molecular biology laboratories, Tween 20 can be used in carefully controlled concentrations for applications involving protein handling, biochemical assays, immunological procedures, washing solutions, and laboratory buffer formulations. Its mild non-ionic detergent characteristics make it useful where controlled surfactant activity is required.

The Molecular Biology grade and the specification of DNAase, RNAase, and proteases as not detectable make this reagent suitable for laboratory workflows where the absence of detectable enzymatic contamination is important.

Non-Ionic Surfactant for Molecular Biology

Tween 20, also called Polysorbate 20, is a non-ionic surfactant. Unlike ionic detergents, non-ionic surfactants do not carry a formal electrical charge and are therefore widely used in biological laboratory formulations.

Its water solubility and viscous liquid form allow convenient preparation of laboratory solutions and buffers. Tween 20 may be incorporated into appropriate experimental formulations according to the requirements of the specific laboratory protocol.

Wide Range of Laboratory Applications

Tween 20 Molecular Biology can be used in various laboratory and research applications, including:

• Molecular biology research
• Biochemistry laboratories
• Cell biology research
• Microbiology applications
• Protein-related laboratory procedures
• Immunological assays
• Immunoassay procedures
• Laboratory buffer preparation
• Washing solutions
• Surface tension reduction
• Biochemical research
• Biological sample processing
• Research and development laboratories
• Pharmaceutical research
• Biotechnology laboratories
• Academic and educational laboratories

Physical and Chemical Properties

At 20 °C, Tween 20 Molecular Biology is supplied as a golden yellow, pale yellow viscous liquid. It has a specified pH value of 7 and a density of approximately 1.095 g/cm³.

The material is soluble in water, making it convenient for preparing aqueous laboratory solutions. The specified vapour pressure is less than 1.33 hPa at 20 °C, while the flash point is 110 °C.

The specified density range of 1.1–1.11 at d20°C/4°C, hydroxyl number of approximately 100, and saponification value of 40–50 provide additional product characteristics for laboratory and quality-control reference.

Why Choose Tween 20 Molecular Biology?

• High-quality Tween 20 / Polysorbate 20
• Molecular Biology grade
• Suitable for molecular biology and biochemical research
• CAS No. 9005-64-5
• Molecular formula C58H114O26
• Molecular weight 1227.72 g/mol
• Non-ionic surfactant
• Soluble in water
• Pale yellow to golden yellow viscous liquid
• DNAase/RNAase/Proteases: Not detectable
• Suitable for laboratory buffer and washing solution preparation
• Useful for immunological and biochemical procedures
• Suitable for research and biotechnology laboratories
• 60 Months shelf life
• Suitable for academic, pharmaceutical, and industrial research applications

Frequently Asked Questions (FAQ)

1. What is Tween-20 Molecular Biology?

Tween 20 Molecular Biology is a Molecular Biology grade non-ionic surfactant, also known as Polysorbate-20, used in various molecular biology, biochemical, and laboratory research applications.

2. What is the CAS number of Tween-20?

The CAS number of Tween-20 is 9005-64-5.

3. What is the molecular formula of Tween-20?

The molecular formula is C58H114O26.

4. What is the molecular weight of Tween-20?

The molecular weight is 1227.72 g/mol.

5. What grade is this Tween-20?

This product is supplied as Molecular Biology grade.

6. Is Tween-20 soluble in water?

Yes. The specified product is soluble in water.

7. What is the physical state of Tween-20 at 20 °C?

Tween-20 is a liquid at 20 °C.

8. What is the appearance of Tween-20?

It is described as a pale yellow to golden yellow viscous liquid.

9. What is the pH value of this Tween-20?

The specified pH value is 7.

10. Are DNAase, RNAase, and proteases detectable in this product?

According to the provided specification, DNAase/RNAase/Proteases are not detectable.

11. What is the shelf life of Tween-20 Molecular Biology?

The stated shelf life is 60 months.

12. What is Tween-20 also known as?

Tween-20 is commonly known as Polysorbate-20.

13. Where is Tween-20 Molecular Biology used?

It can be used in molecular biology, biochemistry, cell biology, microbiology, immunological assays, biotechnology, and other laboratory research applications.

14. Is Tween-20 an ionic or non-ionic surfactant?

Tween-20 is a non-ionic surfactant.

Conclusion

Tween-20 Molecular Biology is a versatile non-ionic surfactant / Polysorbate-20 designed for laboratory and molecular biology applications. With CAS No. 9005-64-5, molecular formula C58H114O26, molecular weight 1227.72 g/mol, water solubility, and DNAase/RNAase/Proteases not detectable, it is suitable for a variety of controlled laboratory procedures.

Its applications include molecular biology, biochemistry, biotechnology, immunological assays, buffer preparation, washing solutions, and biological research. With a stated 60-month shelf life, Tween-20 Molecular Biology provides a practical reagent option for research laboratories, academic institutions, pharmaceutical laboratories, biotechnology facilities, and industrial research environments.